You could say the joke (or the performance/rendition of it) flopped.

(of a performer or show) be completely unsuccessful; fail totally.

For example:

Oh man, last night I cracked a joke and noone even smiled at it. The joke totally flopped.

Or

Oh man, last night I cracked a joke and noone even smiled at it. My timing totally flopped.

You could say the joke (or the performance/rendition of it) flopped.

(of a performer or show) be completely unsuccessful; fail totally.

For example:

Oh man, last night I cracked a joke and no one even smiled at it. The joke totally flopped.

Or

Oh man, last night I cracked a joke and noone even smiled at it. My timing totally flopped.