No, using to take is not a natural way of expressing one's college major in AmE. Note also that "college" is not capitalized unless it's part of the formal name of an institution (e.g., "Dartmouth College").
I took physics in college. This means that I enrolled in one or more physics classes, but leaves my major unspecified.
I majored in physics in college. This explicitly identifies what my major was.
I studied physics in college. This is ambiguous and could mean either it was my major or it was just a class I took. The difference would have to be made in the surrounding context.
In short, take is used for individual courses or classes, not for entire courses of study, where you use the verb to major [in]. For example:
Q: Did you take physics in college?
A: I didn't just take physics, I majored in it!