Without negation, does is used to question the truth of a statement, or to add emphasis. For example:
Does she use the wheels? Yes/No/I don't know
In your example, you're not asking about whether someone uses the wheels or not, but who uses the wheels, if anyone does at all. For instance:
Who uses the wheels? I do/she does/Alice does/Bob does
Does can also be used for emphasis in certain variants of English (US, UK, Aus, Canadian). For example:
Alice: "She doesn't use the wheels."
Bob: "Who does use the wheels?"
In this case, the use of does would be accompanied by a pitch movement, to show emphasis, usually with it being pronounced with a higher pitch than the rest of the sentence.