As usual, the choice between a simple past and a past perfect is nothing to do with the objective facts, but to do with the temporal focus.
If you are choosing to focus on the later time when the groups evolve, and to look backwards from them to the split, it is natural to use the past perfect had separated.
If you have no particular temporal focus, and you are presenting the events in the order they happened, you can use the simple past separated.
There's also the point, as Marcin mentioned, that English speakers don't always use the past perfect even when they could, if the temporal relationships are clear.