Is the phrase "my big blue eyes" correct?
If the words big and blue are describing eyes, shouldn't there be an "and" in-between the two?
When is it mandatory to use "and?"
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"My big blue eyes" is correct, because size adjectives ("big" in this case) must come before color adjectives ("blue" in this case).
"and" isn't frequently used when writing several adjectives, you don't need a word to link them, they must be written just one after the other. It could be used when writing coordinate adjectives, but in general a "," (comma) is used to separate them, and the "and" is used just as a one of the rules needed to figure out if they are actually coordinate adjectives, so in that case you can put a comma between them. Examples:
In addition, their breathtakingly cruel and callous actions also led to a tribute plaque.
Obama stands accused of giving stuffy , (comma) cliche-ridden graduation speeches.
As far as I know, there's not a formal definition to describe when it's mandatory.
But here are some cases where an "and" between adjectives is commonly used:
When using "to be" + "both" / "at once":
Salads are both tasty and delicious.
Salads are at once adj1, adj2, adj3 and adj4
When using "such a":
Have you ever seen such a big and thin pizza?