(Perform it) by myself/yourself etc means without the support or company of anybody else.
You have to complete the cross-country hike by yourself in order to test your map-reading skills. (Nobody is allowed to accompany you).
Perform it myself/yourself/himself/herself/ourselves/themselves is a phrase used to emphasise the person/s concerned, not necessarily to exclude others.
If you think it's easy, try it yourself.
Everybody was amazed that I managed to fix the computer myself.
Because there was no doctor on board the aircraft, the air-crew delivered the baby themselves.
You might have said that they delivered the baby by themselves. This would indicate that nobody else assisted them. They alone did it.