1
 There is no enrollment form, membership fee, and restrictions on the 
 frequency of use.

or

 There is no enrollment form, no membership fee, and no restrictions on the 
 frequency of use. 

If we omit "no", does it change the meaning?

On a similar note:

 I have a blue purse and an indigo umbrella

or

 I have a blue purse and indigo umbrella

If we omit a or an, does it change the meaning/Is it gramatically correct?

2

In the positive sentence, it does not significantly change the meaning.

In the negative sentence, if you only put the one "no", you should use "or" instead of "and":

There is no enrollment form, membership fee, or restrictions on the frequency of use.

This mirrors the way OR, NOT, and AND work in logic:

(NOT A) AND (NOT B) AND (NOT C)

is the same as

NOT (A OR B OR C)

Correct language doesn't always follow the rules of mathematical logic, of course.

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