I want to ask a thing make from/of something but I don't know how.
What are they make from?
What are they make of?
what do they make from?
what do they make of?
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It shouldn't be make from/of, it should be made from/of. Made is the past form of make, and since the item you are asking about already exists, you want to ask what it's made from. For this context, there is little or no difference between made from and made of (which is a shortened form of made out of).
Person 1: "What are car tires made out of?"
Person 2: "They are made of rubber."