In written English, in response to:
Tomorrow is Jane's birthday.
What is the difference between:
Oh, it is?
Oh, is it?
Is there a difference in meaning behind the question?
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Both questions express an element of surprise at the news. However, without any other context, either question can be used in both a good or a not so good way to express surprise at the news of an impending birthday.
Either intonation, or additional context is needed for clarification. For example, both could be followed by "Well, I must get her a present." to mean something nice, or both could be followed by "Well, she is getting up there isn't she." to mean something less nice.
However, you should know that one might say
Oh, it is, is it?
Oh, is it, it is?
is hardly if ever heard, and might be understood to be confusion.
From my experience, "Oh, it is?" is much more dependent on intonation than "Oh, is it?" to express meaning.