Incubate the solution at room temperature for 30 min.
Mix by vortexing and let sit for 10 min at room temperature.
Are these two expressions fully synonymous? Can "incubate" be used with solutions that contain no living organisms, or does it always imply that something living is being maintained or propagated under the mentioned conditions, per Wiktionary's sense 1:
(transitive) To brood, raise, or maintain eggs, organisms, or living tissue through the provision of ideal environmental conditions.
Can one use use "incubate for 10 minutes", say, when describing a drug preparation procedure, in which the drug substance is inanimate matter?
As an aside, what are other widely-used alternatives to "let sit for 10 min"?